The Investigation of Anti-bacterial Activity of Holothuria Leucospilota Sea Cucumber Extracts (Body Wall, Guts and White Strings) at Chabahar Bay in Oman Sea

Document Type: Research Paper

Authors

1 Faculty member of Chabahar Maritime and Marine Sciences University

2 Chabahar Maritime and Marine Sciences University, Chabahar, Iran

3 JondiShapour Medical Sciences University, Ahvaz, Iran

Abstract

Echinoderms are independent and quite special branch of world animals. Sea cucumbers are among the strangest members of echinoderm class regarding their structure and physiology. These fauna have been tested with regard to anti-bacterial, anti-fungal, anti-coagulants, anti-virus, cytotoxic, hemolytic and anti-HIV features. In this study, the antibacterial activity of Holothuria leucospilota sea cucumber was investigated. Antibacterial test was done on 8 strains of bacteria (i.e. Staphylococcus epidermidis, Proteus vulgaris, Shigella dysenteriae, Bacillus cereus, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeroginosa) by two methods of disk diffusion and spot test using aqueous-methanol, methanol, chloroform and n-hexane extracts of body wall, guts and white strings of the sea cucumber collected from Chabahar Bay. Growth inhibition zone was not observed in the disk diffusion method. But a concentration of 2000 micrograms per milliliter of aqueous-methanol extracts of body wall, guts, and white strings had static effect on the growth of some bacteria (Inhibition of bacteria growth).These extracts had no static effect on the growth of bacteria, Sh. dysenteria, P. vulgaris, B. cereus, S. epidermidis. The highest influences of the extracts were on E. coli, S. typhimurium, S. aureus, P. aeroginosa bacteria, respectively.

Keywords